Detection of Ralstonia solanacearum Strains with a Quantitative, Multiplex, Real-Time, Fluorogenic PCR (TaqMan) Assay

A fluorogenic (TaqMan) PCR assay was developed to detect Ralstonia solanacearum strains. Two fluorogenic probes were utilized in a multiplex reaction; one broad-range probe (RS) detected all biovars of R. solanacearum, and a second more specific probe (B2) detected only biovar 2A. Amplification of t...

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Main Authors: Weller, S. A., Elphinstone, J. G., Smith, N. C., Boonham, N., Stead, D. E.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2000
Fag:
Plant Microbiology
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC92083/
https://ncbi.nlm.nih.gov/pubmed/10877778
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