Detection of Ralstonia solanacearum Strains with a Quantitative, Multiplex, Real-Time, Fluorogenic PCR (TaqMan) Assay

A fluorogenic (TaqMan) PCR assay was developed to detect Ralstonia solanacearum strains. Two fluorogenic probes were utilized in a multiplex reaction; one broad-range probe (RS) detected all biovars of R. solanacearum, and a second more specific probe (B2) detected only biovar 2A. Amplification of t...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Weller, S. A., Elphinstone, J. G., Smith, N. C., Boonham, N., Stead, D. E.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 2000
Assuntos:	Plant Microbiology
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC92083/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10877778/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.66.7.2853-2858.2000
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