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Use of Length Heterogeneity PCR and Fatty Acid Methyl Ester Profiles To Characterize Microbial Communities in Soil

In length heterogeneity PCR (LH-PCR) a fluorescently labeled primer is used to determine the relative amounts of amplified sequences originating from different microorganisms. Labeled fragments are separated by gel electrophoresis and detected by laser-induced fluorescence with an automated gene seq...

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Autori principali: Ritchie, Nancy J., Schutter, Mary E., Dick, Richard P., Myrold, David D.
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 2000
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC92039/
https://ncbi.nlm.nih.gov/pubmed/10742258
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