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Detection and Identification of Gastrointestinal Lactobacillus Species by Using Denaturing Gradient Gel Electrophoresis and Species-Specific PCR Primers

Denaturing gradient gel electrophoresis (DGGE) of DNA fragments obtained by PCR amplification of the V2-V3 region of the 16S rRNA gene was used to detect the presence of Lactobacillus species in the stomach contents of mice. Lactobacillus isolates cultured from human and porcine gastrointestinal sam...

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Published in:Appl Environ Microbiol
Main Authors: Walter, J., Tannock, G. W., Tilsala-Timisjarvi, A., Rodtong, S., Loach, D. M., Munro, K., Alatossava, T.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 2000
Subjects:
Food Microbiology
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC91821/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10618239/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.66.1.297-303.2000
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