Cloning, Overexpression, and Mutagenesis of the Sporobolomyces salmonicolor AKU4429 Gene Encoding a New Aldehyde Reductase, Which Catalyzes the Stereoselective Reduction of Ethyl 4-Chloro-3-Oxobutanoate to Ethyl (S)-4-Chloro-3-Hydroxybutanoate

We cloned and sequenced the gene encoding an NADPH-dependent aldehyde reductase (ARII) in Sporobolomyces salmonicolor AKU4429, which reduces ethyl 4-chloro-3-oxobutanoate (4-COBE) to ethyl (S)-4-chloro-3-hydroxybutanoate. The ARII gene is 1,032 bp long, is interrupted by four introns, and encodes a...

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Detalhes bibliográficos
Main Authors: Kita, Keiko, Fukura, Takanobu, Nakase, Koh-Ichi, Okamoto, Kenji, Yanase, Hideshi, Kataoka, Michihiko, Shimizu, Sakayu
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 1999
Assuntos:
Genetics and Molecular Biology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC91706/
https://ncbi.nlm.nih.gov/pubmed/10583966
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