Improved Method for Purification of Bacterial DNA from Bovine Milk for Detection of Brucella spp. by PCR

Different methods of extraction of bacterial DNA from bovine milk to improve the direct detection of Brucella by PCR were evaluated. We found that the use of a lysis buffer with high concentrations of Tris, EDTA, and NaCl, high concentrations of sodium dodecyl sulfate and proteinase K, and high temp...

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Dettagli Bibliografici

Pubblicato in:	Appl Environ Microbiol
Autori principali:	Romero, C., Lopez-Goñi, I.
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 1999
Soggetti:	Food Microbiology
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC91561/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10427076/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.65.8.3735-3737.1999
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