Improved Method for Purification of Bacterial DNA from Bovine Milk for Detection of Brucella spp. by PCR
Different methods of extraction of bacterial DNA from bovine milk to improve the direct detection of Brucella by PCR were evaluated. We found that the use of a lysis buffer with high concentrations of Tris, EDTA, and NaCl, high concentrations of sodium dodecyl sulfate and proteinase K, and high temp...
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| Publicado no: | Appl Environ Microbiol |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology (ASM)
1999
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC91561/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10427076/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.65.8.3735-3737.1999 |
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