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Characterization of Methylglyoxal Synthase from Clostridium acetobutylicum ATCC 824 and Its Use in the Formation of 1,2-Propanediol

A gene encoding a putative 150-amino-acid methylglyoxal synthase was identified in Clostridium acetobutylicum ATCC 824. The enzyme was overexpressed in Escherichia coli and purified. Methylglyoxal synthase has a native molecular mass of 60 kDa and an optimum pH of 7.5. The K(m) and V(max) values for...

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Sonraí Bibleagrafaíochta
Main Authors: Huang, Ke-xue, Rudolph, Frederick B., Bennett, George N.
Formáid: Artigo
Teanga:Inglês
Foilsithe: American Society for Microbiology 1999
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC91483/
https://ncbi.nlm.nih.gov/pubmed/10388730
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