Characterization of Methylglyoxal Synthase from Clostridium acetobutylicum ATCC 824 and Its Use in the Formation of 1,2-Propanediol

A gene encoding a putative 150-amino-acid methylglyoxal synthase was identified in Clostridium acetobutylicum ATCC 824. The enzyme was overexpressed in Escherichia coli and purified. Methylglyoxal synthase has a native molecular mass of 60 kDa and an optimum pH of 7.5. The K(m) and V(max) values for...

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Veröffentlicht in:	Appl Environ Microbiol
Hauptverfasser:	Huang, Ke-xue, Rudolph, Frederick B., Bennett, George N.
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1999
Schlagworte:	Physiology and Biotechnology
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC91483/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10388730/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.65.7.3244-3247.1999
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