High-Efficiency Transformation of Rhizobium leguminosarum by Electroporation

Electrotransformation of Rhizobium leguminosarum was successfully carried out with a 15.1-kb plasmid, pMP154 (Cm(r)), containing a nodABC-lacZ fusion by electroporation. The maximum transformation efficiency, 10(8) transformants/μg of DNA, was achieved at a field strength of 14 kV/cm with a pulse of...

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Pubblicato in:	Appl Environ Microbiol
Autori principali:	Garg, Bindu, Dogra, Romesh C., Sharma, Parveen K.
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 1999
Soggetti:	Plant Microbiology
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC91420/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10347085/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.65.6.2802-2804.1999
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