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High-Efficiency Transformation of Rhizobium leguminosarum by Electroporation
Electrotransformation of Rhizobium leguminosarum was successfully carried out with a 15.1-kb plasmid, pMP154 (Cm(r)), containing a nodABC-lacZ fusion by electroporation. The maximum transformation efficiency, 10(8) transformants/μg of DNA, was achieved at a field strength of 14 kV/cm with a pulse of...
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| Main Authors: | , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society for Microbiology
1999
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC91420/ https://ncbi.nlm.nih.gov/pubmed/10347085 |
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