Pullulanase Type I from Fervidobacterium pennavorans Ven5: Cloning, Sequencing, and Expression of the Gene and Biochemical Characterization of the Recombinant Enzyme

The gene encoding the type I pullulanase from the extremely thermophilic anaerobic bacterium Fervidobacterium pennavorans Ven5 was cloned and sequenced in Escherichia coli. The pulA gene from F. pennavorans Ven5 had 50.1% pairwise amino acid identity with pulA from the anaerobic hyperthermophile The...

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Bibliographic Details

Published in:	Appl Environ Microbiol
Main Authors:	Bertoldo, Costanzo, Duffner, Fiona, Jorgensen, Per L., Antranikian, Garabed
Format:	Artigo
Language:	Inglês
Published:	American Society for Microbiology (ASM) 1999
Subjects:	Enzymology and Protein Engineering
Online Access:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC91302/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10224005/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.65.5.2084-2091.1999
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