Pullulanase Type I from Fervidobacterium pennavorans Ven5: Cloning, Sequencing, and Expression of the Gene and Biochemical Characterization of the Recombinant Enzyme

The gene encoding the type I pullulanase from the extremely thermophilic anaerobic bacterium Fervidobacterium pennavorans Ven5 was cloned and sequenced in Escherichia coli. The pulA gene from F. pennavorans Ven5 had 50.1% pairwise amino acid identity with pulA from the anaerobic hyperthermophile The...

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הוצא לאור ב:	Appl Environ Microbiol
Principais autores:	Bertoldo, Costanzo, Duffner, Fiona, Jorgensen, Per L., Antranikian, Garabed
פורמט:	Artigo
שפה:	Inglês
יצא לאור:	American Society for Microbiology (ASM) 1999
נושאים:	Enzymology and Protein Engineering
גישה מקוונת:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC91302/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10224005/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.65.5.2084-2091.1999
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