Purification and Characterization of Gentisate 1,2-Dioxygenases from Pseudomonas alcaligenes NCIB 9867 and Pseudomonas putida NCIB 9869

Two 3-hydroxybenzoate-inducible gentisate 1,2-dioxygenases were purified to homogeneity from Pseudomonas alcaligenes NCIB 9867 (P25X) and Pseudomonas putida NCIB 9869 (P35X), respectively. The estimated molecular mass of the purified P25X gentisate 1,2-dioxygenase was 154 kDa, with a subunit mass of...

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Bibliographic Details
Main Authors: Feng, Yongmei, Khoo, Hoon Eng, Poh, Chit Laa
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 1999
Subjects:
Enzymology and Protein Engineering
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC91127/
https://ncbi.nlm.nih.gov/pubmed/10049846
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