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Mobilization of Chimeric oriT Plasmids by F and R100-1: Role of Relaxosome Formation in Defining Plasmid Specificity
Cleavage at the F plasmid nic site within the origin of transfer (oriT) requires the F-encoded proteins TraY and TraI and the host-encoded protein integration host factor in vitro. We confirm that F TraY, but not F TraM, is required for cleavage at nic in vivo. Chimeric plasmids were constructed whi...
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| Main Authors: | , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
American Society for Microbiology
2000
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC94588/ https://ncbi.nlm.nih.gov/pubmed/10869081 |
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