Antisense RNA Strategies for Metabolic Engineering of Clostridium acetobutylicum

We examined the effectiveness of antisense RNA (as RNA) strategies for metabolic engineering of Clostridium acetobutylicum. Strain ATCC 824(pRD4) was developed to produce a 102-nucleotide asRNA with 87% complementarity to the butyrate kinase (BK) gene. Strain ATCC 824(pRD4) exhibited 85 to 90% lower...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Desai, Ruchir P., Papoutsakis, Eleftherios T.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1999
Assuntos:	Physiology and Biotechnology
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC91126/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10049845/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.65.3.936-945.1999
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