Detection of Shiga-Like Toxin (stx(1) and stx(2)), Intimin (eaeA), and Enterohemorrhagic Escherichia coli (EHEC) Hemolysin (EHEC hlyA) Genes in Animal Feces by Multiplex PCR

A multiplex PCR was developed for the rapid detection of genes encoding Shiga toxins 1 and 2 (stx(1) and stx(2)), intimin (eaeA), and enterohemolysin A (hlyA) in 444 fecal samples derived from healthy and clinically affected cattle, sheep, pigs, and goats. The method involved non-solvent-based extra...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Fagan, Peter K., Hornitzky, Michael A., Bettelheim, Karl A., Djordjevic, Steven P.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1999
Assuntos:	Methods
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC91113/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9925634/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.65.2.868-872.1999
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