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Detection of Shiga-Like Toxin (stx(1) and stx(2)), Intimin (eaeA), and Enterohemorrhagic Escherichia coli (EHEC) Hemolysin (EHEC hlyA) Genes in Animal Feces by Multiplex PCR

A multiplex PCR was developed for the rapid detection of genes encoding Shiga toxins 1 and 2 (stx(1) and stx(2)), intimin (eaeA), and enterohemolysin A (hlyA) in 444 fecal samples derived from healthy and clinically affected cattle, sheep, pigs, and goats. The method involved non-solvent-based extra...

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Bibliografiske detaljer
Main Authors: Fagan, Peter K., Hornitzky, Michael A., Bettelheim, Karl A., Djordjevic, Steven P.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 1999
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC91113/
https://ncbi.nlm.nih.gov/pubmed/9925634
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