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Screening, Nucleotide Sequence, and Biochemical Characterization of an Esterase from Pseudomonas fluorescens with High Activity towards Lactones

A genomic library of Pseudomonas fluorescens DSM 50106 in a λRESIII phage vector was screened in Escherichia coli K-12 for esterase activity by using α-naphthyl acetate and Fast Blue RR. A 3.2-kb DNA fragment was subcloned from an esterase-positive clone and completely sequenced. Esterase EstF1 was...

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Hlavní autoři: Khalameyzer, V., Fischer, I., Bornscheuer, U. T., Altenbuchner, J.
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology 1999
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC91050/
https://ncbi.nlm.nih.gov/pubmed/9925571
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