Screening, Nucleotide Sequence, and Biochemical Characterization of an Esterase from Pseudomonas fluorescens with High Activity towards Lactones

A genomic library of Pseudomonas fluorescens DSM 50106 in a λRESIII phage vector was screened in Escherichia coli K-12 for esterase activity by using α-naphthyl acetate and Fast Blue RR. A 3.2-kb DNA fragment was subcloned from an esterase-positive clone and completely sequenced. Esterase EstF1 was...

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Detaylı Bibliyografya
Asıl Yazarlar: Khalameyzer, V., Fischer, I., Bornscheuer, U. T., Altenbuchner, J.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: American Society for Microbiology 1999
Konular:
Enzymology and Protein Engineering
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC91050/
https://ncbi.nlm.nih.gov/pubmed/9925571
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