In Situ, Real-Time Catabolic Gene Expression: Extraction and Characterization of Naphthalene Dioxygenase mRNA Transcripts from Groundwater

We developed procedures for isolating and characterizing in situ-transcribed mRNA from groundwater microorganisms catabolizing naphthalene at a coal tar waste-contaminated site. Groundwater was pumped through 0.22-μm-pore-size filters, which were then frozen in dry ice-ethanol. RNA was extracted fro...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Wilson, Mark S., Bakermans, Corien, Madsen, Eugene L.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1999
Assuntos:	General Microbial Ecology
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC90986/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9872763/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.65.1.80-87.1999
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