In Situ, Real-Time Catabolic Gene Expression: Extraction and Characterization of Naphthalene Dioxygenase mRNA Transcripts from Groundwater

We developed procedures for isolating and characterizing in situ-transcribed mRNA from groundwater microorganisms catabolizing naphthalene at a coal tar waste-contaminated site. Groundwater was pumped through 0.22-μm-pore-size filters, which were then frozen in dry ice-ethanol. RNA was extracted fro...

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Bibliografiska uppgifter
Huvudupphovsmän: Wilson, Mark S., Bakermans, Corien, Madsen, Eugene L.
Materialtyp: Artigo
Språk:Inglês
Publicerad: American Society for Microbiology 1999
Ämnen:
General Microbial Ecology
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC90986/
https://ncbi.nlm.nih.gov/pubmed/9872763
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