Biochemical Characterization of the Pseudomonas aeruginosa 101/1477 Metallo-β-Lactamase IMP-1 Produced by Escherichia coli

The bla(IMP) gene coding for the IMP-1 metallo-β-lactamase produced by a Pseudomonas aeruginosa clinical isolate (isolate 101/1477) was overexpressed via a T7 expression system in Escherichia coli BL21(DE3), and its product was purified to homogeneity with a final yield of 35 mg/liter of culture. Th...

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Veröffentlicht in:	Antimicrob Agents Chemother
Hauptverfasser:	Laraki, Nezha, Franceschini, Nicola, Rossolini, Gian Maria, Santucci, Pasqualino, Meunier, Cécile, de Pauw, Edwin, Amicosante, Gianfranco, Frère, Jean Marie, Galleni, Moreno
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1999
Schlagworte:	Mechanisms of Resistance
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC89223/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10103197/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aac.43.4.902
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