Biochemical Characterization of the Pseudomonas aeruginosa 101/1477 Metallo-β-Lactamase IMP-1 Produced by Escherichia coli

The bla(IMP) gene coding for the IMP-1 metallo-β-lactamase produced by a Pseudomonas aeruginosa clinical isolate (isolate 101/1477) was overexpressed via a T7 expression system in Escherichia coli BL21(DE3), and its product was purified to homogeneity with a final yield of 35 mg/liter of culture. Th...

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Hlavní autoři: Laraki, Nezha, Franceschini, Nicola, Rossolini, Gian Maria, Santucci, Pasqualino, Meunier, Cécile, de Pauw, Edwin, Amicosante, Gianfranco, Frère, Jean Marie, Galleni, Moreno
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology 1999
Témata:
Mechanisms of Resistance
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC89223/
https://ncbi.nlm.nih.gov/pubmed/10103197
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