PCR-Based Detection and Identification of Burkholderia cepacia Complex Pathogens in Sputum from Cystic Fibrosis Patients

PCR amplification of the recA gene followed by restriction fragment length polymorphism (RFLP) analysis was investigated for the rapid detection and identification of Burkholderia cepacia complex genomovars directly from sputum. Successful amplification of the B. cepacia complex recA gene from cysti...

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Autors principals: McDowell, Andrew, Mahenthiralingam, Eshwar, Moore, John E., Dunbar, Kerstin E. A., Webb, A. Kevin, Dodd, Mary E., Martin, S. Lorraine, Millar, B. Cherie, Scott, Christopher J., Crowe, Mary, Elborn, J. Stuart
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 2001
Matèries:
Bacteriology
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC88532/
https://ncbi.nlm.nih.gov/pubmed/11724828
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.39.12.4247-4255.2001
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