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PCR-Based Detection and Identification of Burkholderia cepacia Complex Pathogens in Sputum from Cystic Fibrosis Patients

PCR amplification of the recA gene followed by restriction fragment length polymorphism (RFLP) analysis was investigated for the rapid detection and identification of Burkholderia cepacia complex genomovars directly from sputum. Successful amplification of the B. cepacia complex recA gene from cysti...

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Autores principales: McDowell, Andrew, Mahenthiralingam, Eshwar, Moore, John E., Dunbar, Kerstin E. A., Webb, A. Kevin, Dodd, Mary E., Martin, S. Lorraine, Millar, B. Cherie, Scott, Christopher J., Crowe, Mary, Elborn, J. Stuart
Formato: Artigo
Lenguaje:Inglês
Publicado: American Society for Microbiology 2001
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC88532/
https://ncbi.nlm.nih.gov/pubmed/11724828
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.39.12.4247-4255.2001
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