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Detection of Ciprofloxacin-Resistant Yersinia pestis by Fluorogenic PCR Using the LightCycler

We have developed a fluorescence resonance energy transfer (FRET)-based assay to detect ciprofloxacin resistant (Cp(r)) mutants of the biothreat agent Yersinia pestis. We selected spontaneous mutants of the attenuated Y. pestis KIM 5 strain that were resistant to a ciprofloxacin (CIP) concentration...

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Autori principali: Lindler, Luther E., Fan, Wei, Jahan, Nazma
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 2001
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC88402/
https://ncbi.nlm.nih.gov/pubmed/11574586
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.39.10.3649-3655.2001
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