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Detection of Ciprofloxacin-Resistant Yersinia pestis by Fluorogenic PCR Using the LightCycler
We have developed a fluorescence resonance energy transfer (FRET)-based assay to detect ciprofloxacin resistant (Cp(r)) mutants of the biothreat agent Yersinia pestis. We selected spontaneous mutants of the attenuated Y. pestis KIM 5 strain that were resistant to a ciprofloxacin (CIP) concentration...
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Médium: | Artigo |
Jazyk: | Inglês |
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American Society for Microbiology
2001
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On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC88402/ https://ncbi.nlm.nih.gov/pubmed/11574586 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.39.10.3649-3655.2001 |
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