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Development of Conventional and Real-Time PCR Assays for Detection of Legionella DNA in Respiratory Specimens

The development and validation of a PCR assay based on the use of new 16S ribosomal DNA (rDNA)-targeted primers to detect Legionella DNA in respiratory specimens are described. The assay was originally developed as conventional PCR followed by electrophoretic detection and was then adapted to Lightc...

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Détails bibliographiques
Publié dans:J Clin Microbiol
Auteurs principaux: Rantakokko-Jalava, Kaisu, Jalava, Jari
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology (ASM) 2001
Sujets:
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC88258/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11474011/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.39.8.2904-2910.2001
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