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Development of Conventional and Real-Time PCR Assays for Detection of Legionella DNA in Respiratory Specimens
The development and validation of a PCR assay based on the use of new 16S ribosomal DNA (rDNA)-targeted primers to detect Legionella DNA in respiratory specimens are described. The assay was originally developed as conventional PCR followed by electrophoretic detection and was then adapted to Lightc...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology
2001
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC88258/ https://ncbi.nlm.nih.gov/pubmed/11474011 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.39.8.2904-2910.2001 |
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