Direct Detection of Legionella Species from Bronchoalveolar Lavage and Open Lung Biopsy Specimens: Comparison of LightCycler PCR, In Situ Hybridization, Direct Fluorescence Antigen Detection, and Culture

We developed a rapid thermocycling, real-time detection (also known as real-time PCR) method for the detection of Legionella species directly from clinical specimens. This method uses the LightCycler (Roche Molecular Biochemicals, Indianapolis, Ind.) and requires approximately 1 to 2 h to perform. B...

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Detalhes bibliográficos

Publicado no:	J Clin Microbiol
Principais autores:	Hayden, R. T., Uhl, J. R., Qian, X., Hopkins, M. K., Aubry, M. C., Limper, A. H., Lloyd, R. V., Cockerill, F. R.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 2001
Assuntos:	Bacteriology
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC88195/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11427579/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.39.7.2618-2626.2001
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