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Multiplex LightCycler PCR Assay for Detection and Differentiation of Bordetella pertussis and Bordetella parapertussis in Nasopharyngeal Specimens

A rapid real-time multiplex PCR assay for detecting and differentiating Bordetella pertussis and Bordetella parapertussis in nasopharyngeal swabs was developed. This assay (LC-PCR-IS) targets the insertion sequences IS481 and IS1001 of B. pertussis and B. parapertussis, respectively, and is performe...

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Detalhes bibliográficos
Main Authors: Sloan, Lynne M., Hopkins, Marlene K., Mitchell, P. Shawn, Vetter, Emily A., Rosenblatt, Jon E., Harmsen, W. Scott, Cockerill, Franklin R., Patel, Robin
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2002
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC120124/
https://ncbi.nlm.nih.gov/pubmed/11773099
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.40.1.96-100.2002
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