Development of a Real-Time PCR Assay for Detection of Toxoplasma gondii in Pig and Mouse Tissues

A highly sensitive and specific method has been developed to reproducibly detect and quantitate Toxoplasma gondii burden in animal tissue samples using T. gondii ITS1-derived primers and a fluorogenic probe via real-time PCR. Assay specificity was confirmed against a panel of DNA samples from T. gon...

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Pubblicato in:	J Clin Microbiol
Autori principali:	Jauregui, L. H., Higgins, J., Zarlenga, D., Dubey, J. P., Lunney, J. K.
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 2001
Soggetti:	Clinical Veterinary Microbiology
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC88090/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11376036/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.39.6.2065-2071.2001
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