Development of a Real-Time PCR Assay for Detection of Toxoplasma gondii in Pig and Mouse Tissues

A highly sensitive and specific method has been developed to reproducibly detect and quantitate Toxoplasma gondii burden in animal tissue samples using T. gondii ITS1-derived primers and a fluorogenic probe via real-time PCR. Assay specificity was confirmed against a panel of DNA samples from T. gon...

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Bibliografiske detaljer
Main Authors: Jauregui, L. H., Higgins, J., Zarlenga, D., Dubey, J. P., Lunney, J. K.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2001
Fag:
Clinical Veterinary Microbiology
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC88090/
https://ncbi.nlm.nih.gov/pubmed/11376036
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.39.6.2065-2071.2001
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