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Type-Specific Detection of Echovirus 30 Isolates Using Degenerate Reverse Transcriptase PCR Primers

Following an approach used to specifically identify polioviruses and enterovirus 71, we have developed reverse transcriptase (RT) PCR primers containing mixed-base residues or deoxyinosine at positions of codon degeneracy. These primers permit specific RT-PCR amplification of echovirus 30 (E30) sequ...

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Autores principales: Kilpatrick, David R., Quay, Jacqueline, Pallansch, Mark A., Oberste, M. Steven
Formato: Artigo
Lenguaje:Inglês
Publicado: American Society for Microbiology 2001
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC87928/
https://ncbi.nlm.nih.gov/pubmed/11283045
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.39.4.1299-1302.2001
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