Rapid Group-, Serotype-, and Vaccine Strain-Specific Identification of Poliovirus Isolates by Real-Time Reverse Transcription-PCR Using Degenerate Primers and Probes Containing Deoxyinosine Residues

We have adapted our previously described poliovirus diagnostic reverse transcription-PCR (RT-PCR) assays to a real-time RT-PCR (rRT-PCR) format. Our highly specific assays and rRT-PCR reagents are designed for use in the WHO Global Polio Laboratory Network for rapid and large-scale identification of...

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Detalhes bibliográficos
Main Authors: Kilpatrick, David R., Yang, Chen-Fu, Ching, Karen, Vincent, Annelet, Iber, Jane, Campagnoli, Ray, Mandelbaum, Mark, De, Lina, Yang, Su-Ju, Nix, Allan, Kew, Olen M.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2009
Assuntos:
Virology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2691077/
https://ncbi.nlm.nih.gov/pubmed/19386844
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.00702-09
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