Serotype-Specific Identification of Polioviruses by PCR Using Primers Containing Mixed-Base or Deoxyinosine Residues at Positions of Codon Degeneracy

We have developed a method for determining the serotypes of poliovirus isolates by PCR. Three sets of serotype-specific antisense PCR-initiating primers (primers seroPV1A, seroPV2A, and seroPV3A) were designed to pair with codons of VP1 amino acid sequences that are conserved within but that differ...

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Bibliografiska uppgifter
Huvudupphovsmän: Kilpatrick, David R., Nottay, Baldev, Yang, Chen-Fu, Yang, Su-Ju, Da Silva, Edson, Peñaranda, Silvia, Pallansch, Mark, Kew, Olen
Materialtyp: Artigo
Språk:Inglês
Publicerad: American Society for Microbiology 1998
Ämnen:
Virology
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC104541/
https://ncbi.nlm.nih.gov/pubmed/9466740
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