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Serotype-Specific Identification of Polioviruses by PCR Using Primers Containing Mixed-Base or Deoxyinosine Residues at Positions of Codon Degeneracy

We have developed a method for determining the serotypes of poliovirus isolates by PCR. Three sets of serotype-specific antisense PCR-initiating primers (primers seroPV1A, seroPV2A, and seroPV3A) were designed to pair with codons of VP1 amino acid sequences that are conserved within but that differ...

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Publicado no:J Clin Microbiol
Principais autores: Kilpatrick, David R., Nottay, Baldev, Yang, Chen-Fu, Yang, Su-Ju, Da Silva, Edson, Peñaranda, Silvia, Pallansch, Mark, Kew, Olen
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1998
Assuntos:
Virology
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC104541/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9466740/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.36.2.352-357.1998
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