Development of a Species-Specific PCR Assay for Detection of Leishmania donovani in Clinical Samples from Patients with Kala-Azar and Post-Kala-Azar Dermal Leishmaniasis

We have developed a PCR assay that is capable of amplifying kinetoplast DNA (kDNA) of Leishmania donovani in a species-specific manner among Old World leishmanias. With Indian strains and isolates of L. donovani the assay was sensitive enough to detect kDNA in an amount equivalent to a single parasi...

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Main Authors: Salotra, Poonam, Sreenivas, G., Pogue, Gregory P., Lee, Nancy, Nakhasi, Hira L., Ramesh, V., Negi, N. S.
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology 2001
Teme:
Parasitology
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC87840/
https://ncbi.nlm.nih.gov/pubmed/11230394
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.39.3.849-854.2001
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