Diagnosis of Kala-Azar by Nested PCR Based on Amplification of the Leishmania Mini-Exon Gene

To diagnose visceral leishmaniasis (kala-azar), we have developed a nested PCR method based on amplification of the mini-exon gene, which is unique and tandomly repeated in the Leishmania genome. Nested PCR was sufficiently sensitive for the detection of DNA in an amount equivalent to a single Leish...

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Detalhes bibliográficos

Publicado no:	J Clin Microbiol
Principais autores:	Katakura, Ken, Kawazu, Shin-Ichiro, Naya, Toshimitsu, Nagakura, Koichi, Ito, Mamoru, Aikawa, Masamichi, Qu, Jing-Qi, Guan, Li-Ren, Zuo, Xin-Pin, Chai, Jun-Jie, Chang, K.-P., Matsumoto, Yoshitsugu
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1998
Assuntos:	Parasitology
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC104997/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9665985/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.36.8.2173-2177.1998
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