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Diagnosis of Kala-Azar by Nested PCR Based on Amplification of the Leishmania Mini-Exon Gene

To diagnose visceral leishmaniasis (kala-azar), we have developed a nested PCR method based on amplification of the mini-exon gene, which is unique and tandomly repeated in the Leishmania genome. Nested PCR was sufficiently sensitive for the detection of DNA in an amount equivalent to a single Leish...

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Published in:J Clin Microbiol
Main Authors: Katakura, Ken, Kawazu, Shin-Ichiro, Naya, Toshimitsu, Nagakura, Koichi, Ito, Mamoru, Aikawa, Masamichi, Qu, Jing-Qi, Guan, Li-Ren, Zuo, Xin-Pin, Chai, Jun-Jie, Chang, K.-P., Matsumoto, Yoshitsugu
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 1998
Subjects:
Parasitology
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC104997/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9665985/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.36.8.2173-2177.1998
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