Rapid-Cycle PCR for Detection and Typing of Mycoplasma pneumoniae in Clinical Specimens

We designed several new primers and modified previously described species- and type-specific primers targeting the Mycoplasma pneumoniae P1 adhesin gene. Optimized thermal profiles allowed one-step or nested PCR to be completed in less than 1 h. In 10 patients with pneumonia, M. pneumoniae type 1 wa...

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Main Authors: Kong, Fanrong, Gordon, Susanna, Gilbert, Gwendolyn L.
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology 2000
Teme:
Bacteriology
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC87576/
https://ncbi.nlm.nih.gov/pubmed/11060103
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