Rapid-Cycle PCR for Detection and Typing of Mycoplasma pneumoniae in Clinical Specimens

We designed several new primers and modified previously described species- and type-specific primers targeting the Mycoplasma pneumoniae P1 adhesin gene. Optimized thermal profiles allowed one-step or nested PCR to be completed in less than 1 h. In 10 patients with pneumonia, M. pneumoniae type 1 wa...

Ausführliche Beschreibung

Bibliografische Detailangaben

Veröffentlicht in:	J Clin Microbiol
Hauptverfasser:	Kong, Fanrong, Gordon, Susanna, Gilbert, Gwendolyn L.
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 2000
Schlagworte:	Bacteriology
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC87576/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11060103/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.38.11.4256-4259.2000
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