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PCR Detection of Escherichia coli O157:H7 Directly from Stools: Evaluation of Commercial Extraction Methods for Purifying Fecal DNA

Rapid identification of Escherichia coli O157:H7 is important for patient management and for prompt epidemiological investigations. We evaluated one in-house method and three commercially available kits for their ability to extract E. coli O157:H7 DNA directly from stool specimens for PCR. Of the 15...

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Main Authors: Holland, J. L., Louie, L., Simor, A. E., Louie, M.
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology 2000
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC87549/
https://ncbi.nlm.nih.gov/pubmed/11060076
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