PCR Detection of Escherichia coli O157:H7 Directly from Stools: Evaluation of Commercial Extraction Methods for Purifying Fecal DNA

Rapid identification of Escherichia coli O157:H7 is important for patient management and for prompt epidemiological investigations. We evaluated one in-house method and three commercially available kits for their ability to extract E. coli O157:H7 DNA directly from stool specimens for PCR. Of the 15...

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Pubblicato in:	J Clin Microbiol
Autori principali:	Holland, J. L., Louie, L., Simor, A. E., Louie, M.
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 2000
Soggetti:	Bacteriology
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC87549/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11060076/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.38.11.4108-4113.2000
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