Quantitative, Competitive PCR Analysis of Porcine Circovirus DNA in Serum from Pigs with Postweaning Multisystemic Wasting Syndrome

A competitive PCR (cPCR) assay was developed for monitoring porcine circovirus (PCV) DNA in serum samples from piglets. The cPCR was based on competitive coamplification of a 502- or 506-bp region of the PCV type 1 (PCV1) or PCV2 ORF2, respectively, with a known concentration of competitor DNA, whic...

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Bibliographic Details
Main Authors: Liu, Qiang, Wang, Li, Willson, Phil, Babiuk, Lorne A.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2000
Subjects:
Clinical Veterinary Microbiology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC87411/
https://ncbi.nlm.nih.gov/pubmed/10970408
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