Quantitative, Competitive PCR Analysis of Porcine Circovirus DNA in Serum from Pigs with Postweaning Multisystemic Wasting Syndrome

A competitive PCR (cPCR) assay was developed for monitoring porcine circovirus (PCV) DNA in serum samples from piglets. The cPCR was based on competitive coamplification of a 502- or 506-bp region of the PCV type 1 (PCV1) or PCV2 ORF2, respectively, with a known concentration of competitor DNA, whic...

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Podrobná bibliografie

Vydáno v:	J Clin Microbiol
Hlavní autoři:	Liu, Qiang, Wang, Li, Willson, Phil, Babiuk, Lorne A.
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	American Society for Microbiology (ASM) 2000
Témata:	Clinical Veterinary Microbiology
On-line přístup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC87411/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10970408/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.38.9.3474-3477.2000
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