Use of Real-Time PCR and Fluorimetry for Rapid Detection of Rifampin and Isoniazid Resistance-Associated Mutations in Mycobacterium tuberculosis

Very fast amplification of DNA in small volumes can be continuously monitored with a rapid cycler that incorporates fluorimetric detection. Primers were designed to amplify a 157-bp fragment of the rpoB gene spanning codons 526 and 531 and a 209-bp fragment of the katG gene spanning codon 315 of Myc...

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Pubblicato in:	J Clin Microbiol
Autori principali:	Torres, Maria J., Criado, Antonio, Palomares, Jose C., Aznar, Javier
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 2000
Soggetti:	Mycobacteriology and Aerobic Actinomycetes
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC87353/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10970356/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.38.9.3194-3199.2000
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