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Use of Real-Time PCR and Fluorimetry for Rapid Detection of Rifampin and Isoniazid Resistance-Associated Mutations in Mycobacterium tuberculosis

Very fast amplification of DNA in small volumes can be continuously monitored with a rapid cycler that incorporates fluorimetric detection. Primers were designed to amplify a 157-bp fragment of the rpoB gene spanning codons 526 and 531 and a 209-bp fragment of the katG gene spanning codon 315 of Myc...

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Bibliografiska uppgifter
Huvudupphovsmän: Torres, Maria J., Criado, Antonio, Palomares, Jose C., Aznar, Javier
Materialtyp: Artigo
Språk:Inglês
Publicerad: American Society for Microbiology 2000
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Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC87353/
https://ncbi.nlm.nih.gov/pubmed/10970356
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