Detection and Identification of Human Parainfluenza Viruses 1, 2, 3, and 4 in Clinical Samples of Pediatric Patients by Multiplex Reverse Transcription-PCR

We describe a multiplex reverse transcription-PCR (m-RT-PCR) assay that is able to detect and differentiate all known human parainfluenza viruses (HPIVs). Serial dilution experiments with reference strains that compared cell culture isolation and m-RT-PCR showed sensitivities ranging from 0.0004 50%...

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Main Authors: Aguilar, Jose C., Pérez-Breña, María P., García, María L., Cruz, Nieves, Erdman, Dean D., Echevarría, Juan Emilio
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology (ASM) 2000
Teme:
Virology
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC86373/
https://ncbi.nlm.nih.gov/pubmed/10699020
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